LOCUS       40924_41047        3981 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pSpark III, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3981)
  AUTHORS   Paz E.
  TITLE     pSpark III DNA Cloning Vector
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3981)
  AUTHORS   Paz E.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2009) Molecular Biology, Canvax Biotech S.L., 
            Estocolmo, Cordoba, Cordoba 14014, Spain
REFERENCE   3  (bases 1 to 3981)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3981)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (13-AUG-2009) Molecular Biology, Canvax Biotech S.L., Estocolmo, 
            Cordoba, Cordoba 14014, Spain"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3981
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(138..156)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(174..190)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(198..214)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(222..252)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(267..288)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             566..1357
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     rep_origin      complement(1544..2132)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2306..3163)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3164..3268)
                     /label=AmpR promoter
     rep_origin      complement(3346..3801)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     3942..3958
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        3965..3981
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gggcgaattg ggcccccatg ggtttaaacg aattctccgg atcaggcctg atatcaggat
       61 ccactagtgc ggccgcctgc aggtcgactt tcatatgttt gagctcgagt aacgcgttgg
      121 atgcatagct tgagtattct atagtgtcac ctaaatagct tggcgtaatc atggtcatag
      181 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
      241 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
      301 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
      361 cgcgcgggga gaggcggttt gcgtattggg cgctcgcgaa ccggtggttt tatggacagc
      421 aagcgaaccg gaattgccag ctggggcgcc ctctggtaag gttgggaagc cctgcaaagt
      481 aaactggatg gctttcttgc cgccaaggat ctgatggcgc aggggatcaa gctctgatca
      541 agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc
      601 ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc
      661 tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga
      721 cctgtccggt gccctgaatg aactgcaaga cgaggcagcg cggctatcgt ggctggccac
      781 gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct
      841 gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa
      901 agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc
      961 attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct
     1021 tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc
     1081 caggctcaag gcgagcatgc ccgacggcga ggatctcgtc gtgactcatg gcgatgcctg
     1141 cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct
     1201 gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct
     1261 tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca
     1321 gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga agataacgct tcctcgctca
     1381 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     1441 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     1501 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     1561 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     1621 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     1681 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     1741 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     1801 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     1861 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     1921 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     1981 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     2041 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     2101 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     2161 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     2221 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     2281 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     2341 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     2401 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     2461 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     2521 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     2581 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     2641 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     2701 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     2761 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     2821 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     2881 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     2941 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     3001 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     3061 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     3121 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     3181 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     3241 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgatgcgg
     3301 tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggaaatt gtaagcgtta
     3361 atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg
     3421 ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg
     3481 ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa
     3541 aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg
     3601 ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt
     3661 gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg
     3721 ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta
     3781 atgcgccgct acagggcgcg tccattcgcc attcaggctg cgcaactgtt gggaagggcg
     3841 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg
     3901 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga
     3961 attgtaatac gactcactat a
//
